Not known Details About thr77
Not known Details About thr77
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in many tumors, which leads to chromosome instability, and thus may well contribute to tumorigenesis. This protein was located to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial function in selling cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants were described but specific nucleotide sequence info is not yet available.
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Blocking peptides are peptides that bind exclusively towards the focus on antibody and block antibody binding. These peptide generally is made up of the epitope recognized via the antibody.
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This guide outlines even more feasible optimization of cellular and immunoassay parameters to ensure the best possible results are...
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This guideline outlines more attainable optimization of cellular and immunoassay parameters to make certain the very best outcomes are...
Antibodies certain to the blocking peptide now not bind to your epitope to the concentrate on protein. By comparing the staining from your blocked antibody compared to the antibody by yourself, you can see which staining is unique.
Target Details Cyclin E belongs to your remarkably conserved cyclin spouse and children, whose associates are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins exhibit unique expression and degradation patterns which contribute to the temporal coordination of each and every mitotic event. Cyclin E forms a complex with get more info and functions being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates for the G1-S phase boundary which is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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